3D variability analysis reveals a hidden conformational change controlling ammonia transport in human asparagine synthetase
Abstract Advances in X-ray crystallography and cryogenic electron microscopy (cryo-EM) offer the promise of elucidating functionally relevant conformational changes that are not easily studied by other VITAMIN A DROPS biophysical methods.Here we show that 3D variability analysis (3DVA) of the cryo-EM map for wild-type (WT) human asparagine syntheta